NEFM anticorps (phosphospecific)

N° du produit ABIN112358

L’anticorps Souris Monoclonal anti-NEFM a été validé pour WB, IHC (p) et IHC (fro). Il convient pour détecter NEFM dans des échantillons de Humain, Lapin, Singe, Hamster, Xenopus laevis et Cobaye.

Aperçu rapide pour NEFM anticorps (phosphospecific) (ABIN112358)

Antigène: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivité: Humain, Lapin, Singe, Hamster, Xenopus laevis, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RNF406

Détail du produit anti-NEFM anticorps

Épitope: phosphospecific

Specificité: RNF406 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein. Reacts with Human, Rabbit, Hamster, Monkey, Guinea Pig and Xenopus.

Purification: Purified

Immunogène: Neurofilament preparation of calf brain tissue.

Isotype: IgG1

Information d'application

Indications d'application: RNF406 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen sections and paraffin-embedded tissues and Flow cytometry. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilized at 2 - 8 °C and reconstituted at -20 °C.

Détails sur NEFM

Antigène: NEFM (Neurofilament Triplet M Protein (NEFM))

Autre désignation: Neurofilament M (160 kD)

Sujet: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide

ID gène: 4741

NCBI Accession: NP_005373

UniProt: P07197

Pathways: Brown Fat Cell Differentiation